Supersite "Hydrological Nexus of Central Europe" Within the integrated research programs and facilities of the DANUBIUS-CZ consortia (CENAKVA, CZECHGLOBE), we provide to the capacities for the complex investigation of the ongoing processes in freshwater ecosystems and their importance in the terms of biodiversity conservation, protection of aquatic environment, but also protection of water resources for life and human activities. Located in the Czech Republic, the Hydrological Nexus of Central Europe, supersite is a headwater region for European rive systems. It is home to the Elbe, Morava (Danube) and the Odra rivers which support ecosystems, agricalture and transportaition across the whole of Europe. CENAKVA and CZECHGLOBE research facilities specialise in freshwater ecosystems and aquaculture research supporting biodiversity conversation. Services include: water quality and pollution, ekosystem services, biodiversity research and aquaculture innovations. The supersite also offers passive sampling for the detection and observation of polar organic contaminants in the water environment. We connect aquaculturists, hydrobiologist, ecologist, toxicologists and economists studying different factors influencing both water and land ecosystems. This is key to tackling emerging pollutants and nutrients as main contributors to aquatic pollution, helping sustainable water management. This expertise within the supersite provides direct outputs for decision-makers, developing adaptation or mitigation strategies for critical ecosystems.